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Scope of application:

Detection of bovine serum antibody in tuberculosis

Features:

l The world's first reliable ELISA kit for the diagnosis of bovine tuberculosis antibodies

l Experimental methods for the mass detection of M. bovis prior to the intradermal injection of bovine tuberculin: saving time and labor

l A unique combination of antigens that provide a high degree of sensitivity and specificity for test results

l No sample dilution and second wash plate, the test results quickly

1. Prepare the ELISA plate that has been coated with M. burgdorferi antigen

2. Add 50ul of negative control and sample

3. Add 50ul of diluted enzyme to each well

4. Incubate for 60 minutes at 37 ° C

Wash the board 6 times

6. Add 100 ul of substrate to each well and incubate for 15 minutes at room temperature

7. Stop the reaction by adding 100ul stop solution

8. Read the absorbance with dual wavelength, 450nm (dominant wavelength) and 620nm (reference wavelength)

9. S / P = [sample OD value - negative average OD value] / [positive average OD value - negative average OD value]